Ontology highlight
ABSTRACT:
SUBMITTER: Bunemann M
PROVIDER: S-EPMC307695 | biostudies-literature | 2003 Dec
REPOSITORIES: biostudies-literature
Bünemann Moritz M Frank Monika M Lohse Martin J MJ
Proceedings of the National Academy of Sciences of the United States of America 20031212 26
G protein-coupled receptors transduce diverse extracellular signals, such as neurotransmitters, hormones, chemokines, and sensory stimuli, into intracellular responses through activation of heterotrimeric G proteins. G proteins play critical roles in determining specificity and kinetics of subsequent biological responses by modulation of effector proteins. We have developed a fluorescence resonance energy transfer (FRET)-based assay to directly measure mammalian G protein activation in intact ce ...[more]