Ontology highlight
ABSTRACT:
SUBMITTER: Cannon JR
PROVIDER: S-EPMC4222623 | biostudies-literature | 2014 Nov
REPOSITORIES: biostudies-literature
Cannon Joe R JR Holden Dustin D DD Brodbelt Jennifer S JS
Analytical chemistry 20141013 21
We report a hybrid fragmentation method involving electron transfer dissociation (ETD) combined with ultraviolet photodissociation (UVPD) at 193 nm for analysis of intact proteins in an Orbitrap mass spectrometer. Integrating the two fragmentation methods resulted in an increase in the number of identified c- and z-type ions observed when compared to UVPD or ETD alone, as well as generating a more balanced distribution of a/x, b/y, and c/z ion types. Additionally, the method was shown to decreas ...[more]