Ontology highlight
ABSTRACT:
SUBMITTER: Shell TA
PROVIDER: S-EPMC3077068 | biostudies-literature | 2011 Feb
REPOSITORIES: biostudies-literature
Journal of the American Chemical Society 20110128 7
Photolysis of hydroxocobalamin in the presence of plasmid DNA (pBR322) results in DNA cleavage. Temporal control of hydroxyl radical production and DNA strand scission by hydroxocobalamin was demonstrated using a 2-deoxyribose assay and a plasmid relaxation assay, respectively. The light-driven hydroxocobalamin-mediated catalytic formation of hydroxyl radicals was demonstrated using radical scavenging studies of DNA cleavage and via recycling of a hydroxocobalamin-resin conjugate several times w ...[more]