ACGH.Spline--an R package for aCGH dye bias normalization.
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ABSTRACT:
SUBMITTER: Fitzgerald TW
PROVIDER: S-EPMC3077069 | biostudies-literature | 2011 May
REPOSITORIES: biostudies-literature
Publications
aCGH.Spline--an R package for aCGH dye bias normalization.
Fitzgerald Tomas W TW Larcombe Lee D LD Le Scouarnec Solena S Clayton Stephen S Rajan Diana D Carter Nigel P NP Redon Richard R
Bioinformatics (Oxford, England) 20110225 9
<h4>Motivation</h4>The careful normalization of array-based comparative genomic hybridization (aCGH) data is of critical importance for the accurate detection of copy number changes. The difference in labelling affinity between the two fluorophores used in aCGH-usually Cy5 and Cy3-can be observed as a bias within the intensity distributions. If left unchecked, this bias is likely to skew data interpretation during downstream analysis and lead to an increased number of false discoveries.<h4>Resul ...[more]
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