Artificial modulation of the gating behavior of a K+ channel in a KvAP-DNA chimera.
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ABSTRACT:
SUBMITTER: Wang A
PROVIDER: S-EPMC3079724 | biostudies-literature | 2011
REPOSITORIES: biostudies-literature
Publications
Artificial modulation of the gating behavior of a K+ channel in a KvAP-DNA chimera.
Wang Andrew A Zocchi Giovanni G
PloS one 20110419 4
We present experiments where the gating behavior of a voltage-gated ion channel is modulated by artificial ligand binding. We construct a channel-DNA chimera with the KvAP potassium channel reconstituted in an artificial membrane. The channel is functional and the single channel ion conductivity unperturbed by the presence of the DNA. However, the channel opening probability vs. bias voltage, i.e., the gating, can be shifted considerably by the electrostatic force between the charges on the DNA ...[more]
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