Ontology highlight
ABSTRACT:
SUBMITTER: Zhang F
PROVIDER: S-EPMC3084533 | biostudies-literature | 2011 Feb
REPOSITORIES: biostudies-literature
Zhang Feng F Cong Le L Lodato Simona S Kosuri Sriram S Church George M GM Arlotta Paola P
Nature biotechnology 20110119 2
The ability to direct functional proteins to specific DNA sequences is a long-sought goal in the study and engineering of biological processes. Transcription activator-like effectors (TALEs) from Xanthomonas sp. are site-specific DNA-binding proteins that can be readily designed to target new sequences. Because TALEs contain a large number of repeat domains, it can be difficult to synthesize new variants. Here we describe a method that overcomes this problem. We leverage codon degeneracy and typ ...[more]