High affinity scFv-hapten pair as a tool for quantum dot labeling and tracking of single proteins in live cells.
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ABSTRACT:
SUBMITTER: Iyer G
PROVIDER: S-EPMC3084663 | biostudies-literature | 2008 Dec
REPOSITORIES: biostudies-literature
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High affinity scFv-hapten pair as a tool for quantum dot labeling and tracking of single proteins in live cells.
Iyer Gopal G Michalet Xavier X Chang Yun-Pei YP Pinaud Fabien F FF Matyas Stephanie E SE Payne Gregory G Weiss Shimon S
Nano letters 20081201 12
We describe a general approach to label cell surface proteins using quantum dots (QD) for single-molecule tracking. QDs coated with small-hapten modified peptides are targeted to cell surface fusion proteins containing the corresponding single-chain fragment antibody (scFv). The approach is illustrated with the small hapten fluorescein (FL) and a high-affinity anti-FL scFv fused to two different proteins in yeast and murine neuronal cell line N2a. ...[more]
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