Ontology highlight
ABSTRACT:
SUBMITTER: Ashcroft FJ
PROVIDER: S-EPMC3086628 | biostudies-literature | 2011 May
REPOSITORIES: biostudies-literature
Ashcroft F J FJ Newberg J Y JY Jones E D ED Mikic I I Mancini M A MA
Gene 20110120 1-2
Estrogen receptor-α (ER) is an important target both for therapeutic compounds and endocrine disrupting chemicals (EDCs); however, the mechanisms involved in chemical modulation of regulating ER transcriptional activity are inadequately understood. Here, we report the development of a high content analysis-based assay to describe ER activity that uniquely exploits a microscopically visible multi-copy integration of an ER-regulated promoter. Through automated single-cell analyses, we simultaneous ...[more]