Other

Dataset Information

0

High-Content Imaging-Based Pooled CRISPR Screens in Mammalian Cells


ABSTRACT: We developed a microscopy-based approach, which we name optical enrichment, to select cells displaying a particular CRISPR-induced phenotype by automated imaging-based computation, mark them by photo-activation of an expressed photo-activatable fluorescent protein, and then isolate the fluorescent cells using fluorescence-activated cell sorting (FACS). One proof-of-principle screen, a nuclear size screen, a FSC screen and a H2B-mGFP screen were performed to validate the method.

ORGANISM(S): Homo sapiens

PROVIDER: GSE156623 | GEO | 2021/01/12

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2015-11-18 | PXD002959 | Pride
2022-03-06 | GSE197666 | GEO
2024-06-22 | PXD048452 | Pride
2019-10-01 | GSE132704 | GEO
2020-06-28 | GSE151137 | GEO
2018-11-22 | GSE116011 | GEO
| 2317179 | ecrin-mdr-crc
2012-08-07 | E-GEOD-37524 | biostudies-arrayexpress
2024-02-05 | GSE254034 | GEO
2021-07-03 | E-MTAB-9407 | biostudies-arrayexpress