Ontology highlight
ABSTRACT:
SUBMITTER: Bai H
PROVIDER: S-EPMC3088605 | biostudies-literature | 2011 May
REPOSITORIES: biostudies-literature
Proceedings of the National Academy of Sciences of the United States of America 20110418 18
Structural and topological data suggest that serine site-specific DNA recombinases exchange duplex DNAs by rigid-body relative rotation of the two halves of the synapse, mediated by a flat protein-protein interaction surface. We present evidence for this rotational motion for a simple serine recombinase, the Bxb1 phage integrase, from a single-DNA-based supercoil-release assay that allows us to follow crossover site cleavage, rotation, religation, and product release in real time. We have also u ...[more]