Ontology highlight
ABSTRACT:
SUBMITTER: Pena A
PROVIDER: S-EPMC3089579 | biostudies-literature | 2011 May
REPOSITORIES: biostudies-literature
Peña Alejandro A Ripoll-Rozada Jorge J Zunzunegui Sandra S Cabezón Elena E de la Cruz Fernando F Arechaga Ignacio I
The Journal of biological chemistry 20110324 19
Type IV secretion systems (T4SS) mediate the transfer of DNA and protein substrates to target cells. TrwK, encoded by the conjugative plasmid R388, is a member of the VirB4 family, comprising the largest and most conserved proteins of T4SS. In a previous work we demonstrated that TrwK is able to hydrolyze ATP. Here, based on the structural homology of VirB4 proteins with the DNA-pumping ATPase TrwB coupling protein, we generated a series of variants of TrwK where fragments of the C-terminal doma ...[more]