Related B cell clones that populate the CSF and CNS of patients with multiple sclerosis produce CSF immunoglobulin.

ABSTRACT: We investigated the overlap shared between the immunoglobulin (Ig) proteome of the cerebrospinal fluid (CSF) and the B cell Ig-transcriptome of CSF and the central nervous system (CNS) tissue of three patients with multiple sclerosis. We determined the IgG-proteomes of CSF by mass spectrometry, and compared them to the IgG-transcriptomes from CSF and brain lesions, which were analyzed by cDNA cloning. Characteristic peptides that were identified in the CSF-proteome could also be detected in the transcriptomes of both, brain lesions and CSF, providing evidence for a strong overlap of the IgG repertoires in brain lesions and in the CSF.

SUBMITTER: Obermeier B 

PROVIDER: S-EPMC3090654 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature