Ontology highlight
ABSTRACT:
SUBMITTER: Rankin SA
PROVIDER: S-EPMC3092863 | biostudies-literature | 2011 Jun
REPOSITORIES: biostudies-literature
Rankin Scott A SA Zorn Aaron M AM Buchholz Daniel R DR
Developmental dynamics : an official publication of the American Association of Anatomists 20110412 6
We have characterized two new transgenic Xenopus lines enabling transgene expression using the Tet-On inducible system. An inducer line expresses the doxycycline- (Dox-) activated transcription factor rtTA under control of the ubiquitous promoter CMV. A responder line enables Dox-inducible expression of a dominant positive thyroid hormone receptor via a tetracycline responsive transgenic promoter (TRE). Dox-induced expression of transgenic GFP mRNA was detectable after 3 hr and increased up to 1 ...[more]