Ontology highlight
ABSTRACT:
SUBMITTER: Pawlak M
PROVIDER: S-EPMC3093119 | biostudies-literature | 2011 May
REPOSITORIES: biostudies-literature
Pawlak Mathias M Jaenisch Rudolf R
Genes & development 20110501 10
Induced pluripotent stem cells (iPSCs) are generated from somatic cells by the transduction of defined transcription factors, and this process involves dynamic changes in DNA methylation. While the reprogramming of somatic cells is accompanied by demethylation of pluripotency genes, the functional importance of de novo DNA methylation has not been clarified. Here, using loss-of-function studies, we generated iPSCs from fibroblasts that were deficient in de novo DNA methylation mediated by Dnmt3a ...[more]