Ontology highlight
ABSTRACT:
SUBMITTER: Hodeify R
PROVIDER: S-EPMC3094048 | biostudies-literature | 2011 May
REPOSITORIES: biostudies-literature
Hodeify Rawad R Tarcsafalvi Adel A Megyesi Judit J Safirstein Robert L RL Price Peter M PM
American journal of physiology. Renal physiology 20110216 5
Cisplatin cytotoxicity is dependent on cyclin-dependent kinase 2 (Cdk2) activity in vivo and in vitro. We found that an 18-kDa protein identified by mass spectrometry as p21(WAF1/Cip1) was phosphorylated by Cdk2 starting 12 h after cisplatin exposure. The analysis showed it was phosphorylated at serine 78, a site not previously identified. The adenoviral transduction of p21 before cisplatin exposure protects from cytotoxicity by inhibiting Cdk2. Although cisplatin causes induction of endogenous ...[more]