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Preparation and analysis of glycan microarrays.

ABSTRACT: Determination of the binding specificity of glycan-binding proteins (GBPs), such as lectins, antibodies, and receptors, has traditionally been difficult and laborious. The advent of glycan microarrays has revolutionized the field of glycobiology by allowing simultaneous screening of a GBP for interactions with a large set of glycans in a single format. This unit describes the theory and method for production of two types of glycan microarrays (chemo/enzymatically synthesized and naturally derived), and their application to functional glycomics to explore glycan recognition by GBPs. These procedures are amenable to various types of arrays and a wide range of GBP samples.

SUBMITTER: Heimburg-Molinaro J

PROVIDER: S-EPMC3097418 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature

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Preparation and analysis of glycan microarrays.

Heimburg-Molinaro Jamie J Song Xuezheng X Smith David F DF Cummings Richard D RD

Current protocols in protein science 20110401

Determination of the binding specificity of glycan-binding proteins (GBPs), such as lectins, antibodies, and receptors, has traditionally been difficult and laborious. The advent of glycan microarrays has revolutionized the field of glycobiology by allowing simultaneous screening of a GBP for interactions with a large set of glycans in a single format. This unit describes the theory and method for production of two types of glycan microarrays (chemo/enzymatically synthesized and naturally derive ...[more]

PMID: 21488041

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