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Rapid identification of bacteria with a disposable colorimetric sensing array.

ABSTRACT: Rapid identification of both species and even specific strains of human pathogenic bacteria grown on standard agar has been achieved from the volatiles they produce using a disposable colorimetric sensor array in a Petri dish imaged with an inexpensive scanner. All 10 strains of bacteria tested, including Enterococcus faecalis and Staphylococcus aureus and their antibiotic-resistant forms, were identified with 98.8% accuracy within 10 h, a clinically important time frame. Furthermore, the colorimetric sensor arrays also proved useful as a simple research tool for the study of bacterial metabolism and as an easy method for the optimization of bacterial production of fine chemicals or other fermentation processes.

SUBMITTER: Carey JR

PROVIDER: S-EPMC3097425 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature

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Rapid identification of bacteria with a disposable colorimetric sensing array.

Carey James R JR Suslick Kenneth S KS Hulkower Keren I KI Imlay James A JA Imlay Karin R C KR Ingison Crystal K CK Ponder Jennifer B JB Sen Avijit A Wittrig Aaron E AE

Journal of the American Chemical Society 20110427 19

Rapid identification of both species and even specific strains of human pathogenic bacteria grown on standard agar has been achieved from the volatiles they produce using a disposable colorimetric sensor array in a Petri dish imaged with an inexpensive scanner. All 10 strains of bacteria tested, including Enterococcus faecalis and Staphylococcus aureus and their antibiotic-resistant forms, were identified with 98.8% accuracy within 10 h, a clinically important time frame. Furthermore, the colori ...[more]

PMID: 21524080

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