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Colorimetric bacteria sensing using a supramolecular enzyme-nanoparticle biosensor.

ABSTRACT: Rapid and sensitive detection of pathogens is a key requirement for both environmental and clinical settings. We report here a colorimetric enzyme-nanoparticle conjugate system for detection of microbial contamination. In this approach, cationic gold nanoparticles (NPs) featuring quaternary amine headgroups are electrostatically bound to an enzyme [?-galactosidase (?-Gal)], inhibiting enzyme activity. Analyte bacteria bind to the NP, which releases the ?-Gal and restores its activity, providing an enzyme-amplified colorimetric readout of the binding event. Using this strategy, we have been able to quantify bacteria at concentrations of 1 × 10(2) bacteria/mL in solution and 1 × 10(4) bacteria/mL in a field-friendly test strip format.

SUBMITTER: Miranda OR

PROVIDER: S-EPMC3120917 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature

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Colorimetric bacteria sensing using a supramolecular enzyme-nanoparticle biosensor.

Miranda Oscar R OR Li Xiaoning X Garcia-Gonzalez Limary L Zhu Zheng-Jiang ZJ Yan Bo B Bunz Uwe H F UH Rotello Vincent M VM

Journal of the American Chemical Society 20110607 25

Rapid and sensitive detection of pathogens is a key requirement for both environmental and clinical settings. We report here a colorimetric enzyme-nanoparticle conjugate system for detection of microbial contamination. In this approach, cationic gold nanoparticles (NPs) featuring quaternary amine headgroups are electrostatically bound to an enzyme [β-galactosidase (β-Gal)], inhibiting enzyme activity. Analyte bacteria bind to the NP, which releases the β-Gal and restores its activity, providing ...[more]

PMID: 21627131

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