Ontology highlight
ABSTRACT:
SUBMITTER: Stevenson L
PROVIDER: S-EPMC3101240 | biostudies-literature | 2011
REPOSITORIES: biostudies-literature
Stevenson Leanne L Allen Wendy L WL Proutski Irina I Stewart Gail G Johnston Louise L McCloskey Karen K Wilson Peter M PM Longley Daniel B DB Johnston Patrick G PG
PloS one 20110524 5
The role of the calcium binding protein, Calbindin 2 (CALB2), in regulating the response of colorectal cancer (CRC) cells to 5-Fluorouracil (5-FU) was investigated. Real-time RT-PCR and Western blot analysis revealed that CALB2 mRNA and protein expression were down-regulated in p53 wild-type and p53 null isogenic HCT116 CRC cell lines following 48 h and 72 h 5-FU treatment. Moreover, 5-FU-induced apoptosis was significantly reduced in HCT116 and LS174T CRC cell lines in which CALB2 expression ha ...[more]