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Rapid and enhanced proteolytic digestion using electric-field-oriented enzyme reactor.

ABSTRACT: We have created a novel enzyme reactor using electric field-mediated orientation and immobilization of proteolytic enzymes (trypsin/chymotrypsin) on biocompatible PVDF membranes in a continuous flow-through chamber. Using less than 5min, this reactor in various enzyme combinations can produce enhanced rapid digestion for standardized prototypic proteins, hydrophilic proteins and hydrophobic transmembrane proteins when compared to in-solution techniques. With improved digestive efficiency, our reactor improved the overall functional analysis of lipid raft proteomes by identifying more closely functionally linked proteins and elucidated a richer set of biological processes and pathways linked to the proteins than traditional in-solution methods.

SUBMITTER: Zhou Y

PROVIDER: S-EPMC3102135 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature

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Rapid and enhanced proteolytic digestion using electric-field-oriented enzyme reactor.

Zhou Yu Y Yi Tie T Park Sung-Soo SS Chadwick Wayne W Shen Rong-Fong RF Wu Wells W WW Martin Bronwen B Maudsley Stuart S

Journal of proteomics 20110219 7

We have created a novel enzyme reactor using electric field-mediated orientation and immobilization of proteolytic enzymes (trypsin/chymotrypsin) on biocompatible PVDF membranes in a continuous flow-through chamber. Using less than 5min, this reactor in various enzyme combinations can produce enhanced rapid digestion for standardized prototypic proteins, hydrophilic proteins and hydrophobic transmembrane proteins when compared to in-solution techniques. With improved digestive efficiency, our re ...[more]

PMID: 21338726

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