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GATE-16, a membrane transport modulator, interacts with NSF and the Golgi v-SNARE GOS-28.

ABSTRACT: Membrane proteins located on vesicles (v-SNAREs) and on the target membrane (t-SNAREs) mediate specific recognition and, possibly, fusion between a transport vesicle and its target membrane. The activity of SNARE molecules is regulated by several soluble cytosolic proteins. We have cloned a bovine brain cDNA encoding a conserved 117 amino acid polypeptide, denoted Golgi-associated ATPase Enhancer of 16 kDa (GATE-16), that functions as a soluble transport factor. GATE-16 interacts with N-ethylmaleimidesensitive factor (NSF) and significantly stimulates its ATPase activity. It also interacts with the Golgi v-SNARE GOS-28 in an NSF-dependent manner. We propose that GATE-16 modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation.

SUBMITTER: Sagiv Y

PROVIDER: S-EPMC310219 | biostudies-literature | 2000 Apr

REPOSITORIES: biostudies-literature

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GATE-16, a membrane transport modulator, interacts with NSF and the Golgi v-SNARE GOS-28.

Sagiv Y Y Legesse-Miller A A Porat A A Elazar Z Z

The EMBO journal 20000401 7

Membrane proteins located on vesicles (v-SNAREs) and on the target membrane (t-SNAREs) mediate specific recognition and, possibly, fusion between a transport vesicle and its target membrane. The activity of SNARE molecules is regulated by several soluble cytosolic proteins. We have cloned a bovine brain cDNA encoding a conserved 117 amino acid polypeptide, denoted Golgi-associated ATPase Enhancer of 16 kDa (GATE-16), that functions as a soluble transport factor. GATE-16 interacts with N-ethylmal ...[more]

PMID: 10747018

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