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Proteomics-based discovery of koranimine, a cyclic imine natural product.

ABSTRACT: Nonribosomal peptide synthetases (NRPSs) and polyketide synthases (PKSs) are large enzymes responsible for the biosynthesis of medically and ecologically important secondary metabolites. In a previous report, we described a proteomics approach to screen for expressed NRPSs or PKSs from bacteria with or without sequenced genomes. Here we used this proteome mining approach to discover a novel natural product arising from rare adenylation (A) and reductase (Red) domains in its biosynthetic machinery. We also cloned the entire gene cluster and elucidated the biosynthesis of the new compound, which is produced by an unsequenced Bacillus sp. isolated from soil collected in Koran, Louisiana.

SUBMITTER: Evans BS

PROVIDER: S-EPMC3103991 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature

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Proteomics-based discovery of koranimine, a cyclic imine natural product.

Evans Bradley S BS Ntai Ioanna I Chen Yunqiu Y Robinson Sarah J SJ Kelleher Neil L NL

Journal of the American Chemical Society 20110426 19

Nonribosomal peptide synthetases (NRPSs) and polyketide synthases (PKSs) are large enzymes responsible for the biosynthesis of medically and ecologically important secondary metabolites. In a previous report, we described a proteomics approach to screen for expressed NRPSs or PKSs from bacteria with or without sequenced genomes. Here we used this proteome mining approach to discover a novel natural product arising from rare adenylation (A) and reductase (Red) domains in its biosynthetic machiner ...[more]

PMID: 21520944

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