Dataset Information
Mir-34a is upregulated during liver regeneration in rats and is associated with the suppression of hepatocyte proliferation.
Ontology highlight
ABSTRACT: Background
MicroRNAs are a class of small regulatory RNAs that modulate a variety of biological processes, including cellular differentiation, apoptosis, metabolism and proliferation. This study aims to explore the effect of miR-34a in hepatocyte proliferation and its potential role in liver regeneration termination.Methodology/principal finding
MiR-34a was highly induced after partial hepatectomy. Overexpression of miR-34a in BRL-3A cells could significantly inhibit cell proliferation and down-regulate the expression of inhibin ?B (INHBB) and Met. In BRL-3A cells, INHBB was identified as a direct target of miR-34a by luciferase reporter assay. More importantly, INHBB siRNA significantly repressed cell proliferation. A decrease of INHBB and Met was detected in regenerating liver.Conclusion/significance
MiR-34a expression was upregulated during the late phase of liver regeneration. MiR-34a-mediated regulation of INHBB and Met may collectively contribute to the suppression of hepatocyte proliferation.
SUBMITTER: Chen H
PROVIDER: S-EPMC3105003 | biostudies-literature | 2011
REPOSITORIES: biostudies-literature
Publications
Mir-34a is upregulated during liver regeneration in rats and is associated with the suppression of hepatocyte proliferation.
PloS one 20110531 5
<h4>Background</h4>MicroRNAs are a class of small regulatory RNAs that modulate a variety of biological processes, including cellular differentiation, apoptosis, metabolism and proliferation. This study aims to explore the effect of miR-34a in hepatocyte proliferation and its potential role in liver regeneration termination.<h4>Methodology/principal finding</h4>MiR-34a was highly induced after partial hepatectomy. Overexpression of miR-34a in BRL-3A cells could significantly inhibit cell prolife ...[more]
