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Crystallization and preliminary X-ray studies of a galactose-specific lectin from the seeds of Spatholobus parviflorus.

ABSTRACT: A galactose-specific seed lectin was purified from the legume Spatholobus parviflorus and crystallized using the hanging-drop vapour-diffusion technique. The crystals belonged to space group P1, with unit-cell parameters a = 60.998, b = 60.792, c = 78.179?Å, ? = 101.32, ? = 91.38, ? = 104.32°. X-ray diffraction data were collected under cryoconditions (100?K) to a resolution of 2.04?Å using a MAR image-plate detector system mounted on a rotating-anode X-ray (Cu?K?) generator. Molecular replacement using legume-lectin coordinates as a search model gave a tetrameric structure.

SUBMITTER: Geethanandan K

PROVIDER: S-EPMC3107147 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature

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Crystallization and preliminary X-ray studies of a galactose-specific lectin from the seeds of Spatholobus parviflorus.

Geethanandan K K Abhilash Joseph J Bharath S R SR Sadasivan C C Haridas M M

Acta crystallographica. Section F, Structural biology and crystallization communications 20110526 Pt 6

A galactose-specific seed lectin was purified from the legume Spatholobus parviflorus and crystallized using the hanging-drop vapour-diffusion technique. The crystals belonged to space group P1, with unit-cell parameters a = 60.998, b = 60.792, c = 78.179 Å, α = 101.32, β = 91.38, γ = 104.32°. X-ray diffraction data were collected under cryoconditions (100 K) to a resolution of 2.04 Å using a MAR image-plate detector system mounted on a rotating-anode X-ray (Cu Kα) generator. Molecular replaceme ...[more]

PMID: 21636916

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