Ontology highlight
ABSTRACT:
SUBMITTER: Fu GK
PROVIDER: S-EPMC3107322 | biostudies-literature | 2011 May
REPOSITORIES: biostudies-literature
Fu Glenn K GK Hu Jing J Wang Pei-Hua PH Fodor Stephen P A SP
Proceedings of the National Academy of Sciences of the United States of America 20110511 22
We implement a unique strategy for single molecule counting termed stochastic labeling, where random attachment of a diverse set of labels converts a population of identical DNA molecules into a population of distinct DNA molecules suitable for threshold detection. The conceptual framework for stochastic labeling is developed and experimentally demonstrated by determining the absolute and relative number of selected genes after stochastically labeling approximately 360,000 different fragments of ...[more]