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Sequencing of cDNA clones from the genetic map of tomato (Lycopersicon esculentum).

ABSTRACT: The dense RFLP linkage map of tomato (Lycopersicon esculentum) contains >300 anonymous cDNA clones. Of those clones, 272 were partially or completely sequenced. The sequences were compared at the DNA and protein level to known genes in databases. For 57% of the clones, a significant match to previously described genes was found. The information will permit the conversion of those markers to STS markers and allow their use in PCR-based mapping experiments. Furthermore, it will facilitate the comparative mapping of genes across distantly related plant species by direct comparison of DNA sequences and map positions. [cDNA sequence data reported in this paper have been submitted to the EMBL database under accession nos. AA824695-AA825005 and the dbEST_Id database under accession nos. 1546519-1546862.]

SUBMITTER: Ganal MW

PROVIDER: S-EPMC310761 | biostudies-literature | 1998 Aug

REPOSITORIES: biostudies-literature

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Sequencing of cDNA clones from the genetic map of tomato (Lycopersicon esculentum).

Ganal M W MW Czihal R R Hannappel U U Kloos D U DU Polley A A Ling H Q HQ

Genome research 19980801 8

The dense RFLP linkage map of tomato (Lycopersicon esculentum) contains >300 anonymous cDNA clones. Of those clones, 272 were partially or completely sequenced. The sequences were compared at the DNA and protein level to known genes in databases. For 57% of the clones, a significant match to previously described genes was found. The information will permit the conversion of those markers to STS markers and allow their use in PCR-based mapping experiments. Furthermore, it will facilitate the comp ...[more]

PMID: 9724330

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