Implications of molecular heterogeneity for the cooperativity of biological macromolecules.
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ABSTRACT:
SUBMITTER: Solomatin SV
PROVIDER: S-EPMC3109240 | biostudies-literature | 2011 Jun
REPOSITORIES: biostudies-literature
Publications
Implications of molecular heterogeneity for the cooperativity of biological macromolecules.
Solomatin Sergey V SV Greenfeld Max M Herschlag Daniel D
Nature structural & molecular biology 20110515 6
Cooperativity, a universal property of biological macromolecules, is typically characterized by a Hill slope, which can provide fundamental information about binding sites and interactions. We demonstrate, through simulations and single-molecule FRET (smFRET) experiments, that molecular heterogeneity lowers bulk cooperativity from the intrinsic value for the individual molecules. As heterogeneity is common in smFRET experiments, appreciation of its influence on fundamental measures of cooperativ ...[more]
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