A Stochastic Kinematic Model of Class Averaging in Single-Particle Electron Microscopy.

ABSTRACT: Single-particle electron microscopy is an experimental technique that is used to determine the 3D structure of biological macromolecules and the complexes that they form. In general, image processing techniques and reconstruction algorithms are applied to micrographs, which are two-dimensional (2D) images taken by electron microscopes. Each of these planar images can be thought of as a projection of the macromolecular structure of interest from an a priori unknown direction. A class is defined as a collection of projection images with a high degree of similarity, presumably resulting from taking projections along similar directions. In practice, micrographs are very noisy and those in each class are aligned and averaged in order to reduce the background noise. Errors in the alignment proce

SUBMITTER: Park W 

PROVIDER: S-EPMC3110017 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature