Advances in Single-Particle Electron Cryomicroscopy Structure Determination applied to Sub-tomogram Averaging.

ABSTRACT: Recent innovations in specimen preparation, data collection, and image processing have led to improved structure determination using single-particle electron cryomicroscopy (cryo-EM). Here we explore some of these advances to improve structures determined using electron cryotomography (cryo-ET) and sub-tomogram averaging. We implement a new three-dimensional model for the contrast transfer function, and use this in a regularized likelihood optimization algorithm as implemented in the RELION program. Using direct electron detector data, we apply both single-particle analysis and sub-tomogram averaging to analyze radiation-induced movements of the specimen. As in single-particle cryo-EM, we find that significant sample movements occur during tomographic data acquisition, and that these movem

SUBMITTER: Bharat TAM 

PROVIDER: S-EPMC4559595 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature