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Redox biology: computational approaches to the investigation of functional cysteine residues.

ABSTRACT: Cysteine (Cys) residues serve many functions, such as catalysis, stabilization of protein structure through disulfides, metal binding, and regulation of protein function. Cys residues are also subject to numerous post-translational modifications. In recent years, various computational tools aiming at classifying and predicting different functional categories of Cys have been developed, particularly for structural and catalytic Cys. On the other hand, given complexity of the subject, bioinformatics approaches have been less successful for the investigation of regulatory Cys sites. In this review, we introduce different functional categories of Cys residues. For each category, an overview of state-of-the-art bioinformatics methods and tools is provided, along with examples of successful applications and potential limitations associated with each approach. Finally, we discuss Cys-based redox switches, which modify the view of distinct functional categories of Cys in proteins.

SUBMITTER: Marino SM

PROVIDER: S-EPMC3110093 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature

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Redox biology: computational approaches to the investigation of functional cysteine residues.

Marino Stefano M SM Gladyshev Vadim N VN

Antioxidants & redox signaling 20110414 1

Cysteine (Cys) residues serve many functions, such as catalysis, stabilization of protein structure through disulfides, metal binding, and regulation of protein function. Cys residues are also subject to numerous post-translational modifications. In recent years, various computational tools aiming at classifying and predicting different functional categories of Cys have been developed, particularly for structural and catalytic Cys. On the other hand, given complexity of the subject, bioinformati ...[more]

PMID: 20812876

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