Theories and applications for sequencing randomly selected clones.
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ABSTRACT:
SUBMITTER: Wendl MC
PROVIDER: S-EPMC311021 | biostudies-literature | 2001 Feb
REPOSITORIES: biostudies-literature
Publications
Theories and applications for sequencing randomly selected clones.
Wendl M C MC Marra M A MA Hillier L W LW Chinwalla A T AT Wilson R K RK Waterston R H RH
Genome research 20010201 2
Theory is developed for the process of sequencing randomly selected large-insert clones. Genome size, library depth, clone size, and clone distribution are considered relevant properties and perfect overlap detection for contig assembly is assumed. Genome-specific and nonrandom effects are neglected. Order of magnitude analysis indicates library depth is of secondary importance compared to the other variables, especially as clone size diminishes. In such cases, the well-known Poisson coverage la ...[more]
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