Construction of long-transcript enriched cDNA libraries from submicrogram amounts of total RNAs by a universal PCR amplification method.
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ABSTRACT:
SUBMITTER: Piao Y
PROVIDER: S-EPMC311119 | biostudies-literature | 2001 Sep
REPOSITORIES: biostudies-literature
Publications
Construction of long-transcript enriched cDNA libraries from submicrogram amounts of total RNAs by a universal PCR amplification method.
Piao Y Y Ko N T NT Lim M K MK Ko M S MS
Genome research 20010901 9
Here we report a novel design of linker primer that allows one to differentially amplify long tracts (average 3.0 kb with size ranges of 1-7 kb) or short DNAs (average 1.5 kb with size ranges of 0.5-3 kb) from a complex mixture. The method allows one to generate cDNA libraries enriched for long transcripts without size selection of insert DNAs. One representative library from newborn kidney includes 70% of clones bearing ATG start codons. A comparable library has been generated from 20 mouse bla ...[more]
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