Ontology highlight
ABSTRACT:
SUBMITTER: Xie J
PROVIDER: S-EPMC3111922 | biostudies-literature | 2009 Jun
REPOSITORIES: biostudies-literature
Xie Jingjing J Thapa Rajiv R Reverdatto Sergey S Burz David S DS Shekhtman Alexander A
Journal of medicinal chemistry 20090601 11
We developed an in-cell NMR assay for screening small molecule interactor libraries (SMILI-NMR) for compounds capable of disrupting or enhancing specific interactions between two or more components of a biomolecular complex. The method relies on the formation of a well-defined biocomplex and utilizes in-cell NMR spectroscopy to identify the molecular surfaces involved in the interaction at atomic scale resolution. Changes in the interaction surface caused by a small molecule interfering with com ...[more]