Unknown

Dataset Information

0

Mapping structural interactions using in-cell NMR spectroscopy (STINT-NMR).


ABSTRACT: We describe a high-throughput in-cell nuclear magnetic resonance (NMR)-based method for mapping the structural changes that accompany protein-protein interactions (STINT-NMR). The method entails sequentially expressing two (or more) proteins within a single bacterial cell in a time-controlled manner and monitoring the protein interactions using in-cell NMR spectroscopy. The resulting spectra provide a complete titration of the interaction and define structural details of the interacting surfaces at atomic resolution.

SUBMITTER: Burz DS 

PROVIDER: S-EPMC4447212 | biostudies-literature | 2006 Feb

REPOSITORIES: biostudies-literature

altmetric image

Publications

Mapping structural interactions using in-cell NMR spectroscopy (STINT-NMR).

Burz David S DS   Dutta Kaushik K   Cowburn David D   Shekhtman Alexander A  

Nature methods 20060201 2


We describe a high-throughput in-cell nuclear magnetic resonance (NMR)-based method for mapping the structural changes that accompany protein-protein interactions (STINT-NMR). The method entails sequentially expressing two (or more) proteins within a single bacterial cell in a time-controlled manner and monitoring the protein interactions using in-cell NMR spectroscopy. The resulting spectra provide a complete titration of the interaction and define structural details of the interacting surfaces  ...[more]

Similar Datasets

| S-EPMC2453524 | biostudies-literature
| S-EPMC4041589 | biostudies-literature
| S-EPMC6258002 | biostudies-literature
| S-EPMC6082733 | biostudies-literature
| S-EPMC1304007 | biostudies-other
| S-EPMC2154429 | biostudies-literature
| S-EPMC7039561 | biostudies-literature
| S-EPMC4510928 | biostudies-literature
| S-EPMC3111922 | biostudies-literature
| S-EPMC3796943 | biostudies-literature