Ontology highlight
ABSTRACT:
SUBMITTER: Burz DS
PROVIDER: S-EPMC4447212 | biostudies-literature | 2006 Feb
REPOSITORIES: biostudies-literature
Burz David S DS Dutta Kaushik K Cowburn David D Shekhtman Alexander A
Nature methods 20060201 2
We describe a high-throughput in-cell nuclear magnetic resonance (NMR)-based method for mapping the structural changes that accompany protein-protein interactions (STINT-NMR). The method entails sequentially expressing two (or more) proteins within a single bacterial cell in a time-controlled manner and monitoring the protein interactions using in-cell NMR spectroscopy. The resulting spectra provide a complete titration of the interaction and define structural details of the interacting surfaces ...[more]