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Highly efficient chemoenzymatic synthesis of ?1-3-linked galactosides.

ABSTRACT: A novel D-galactosyl-?1-3-N-acetyl-D-hexosamine phosphorylase cloned from Bifidobacterium infantis (BiGalHexNAcP) was used with a recombinant E. coli K-12 galactokinase (GalK) for efficient one-pot two-enzyme synthesis of T-antigens, galacto-N-biose (Gal?1-3GalNAc), lacto-N-biose (Gal?1-3GlcNAc), and their derivatives.

SUBMITTER: Yu H

PROVIDER: S-EPMC3114946 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature

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Highly efficient chemoenzymatic synthesis of β1-3-linked galactosides.

Yu Hai H Thon Vireak V Lau Kam K Cai Li L Chen Yi Y Mu Shengmao S Li Yanhong Y Wang Peng George PG Chen Xi X

Chemical communications (Cambridge, England) 20100910 40

A novel D-galactosyl-β1-3-N-acetyl-D-hexosamine phosphorylase cloned from Bifidobacterium infantis (BiGalHexNAcP) was used with a recombinant E. coli K-12 galactokinase (GalK) for efficient one-pot two-enzyme synthesis of T-antigens, galacto-N-biose (Galβ1-3GalNAc), lacto-N-biose (Galβ1-3GlcNAc), and their derivatives. ...[more]

PMID: 20830443

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