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Short form of ?9 promotes ?9?1 integrin-dependent cell adhesion by modulating the function of the full-length ?9 subunit.

ABSTRACT: The ?9?1 integrin is a multifunctional receptor that interacts with a variety of ligands including vascular cell adhesion molecule 1, tenascin-C, and osteopontin. A 2.3-kb truncated form of ?9 integrin subunit cDNA was identified by searching the Medline database. This splice variant, which we called the short form of ?9 integrin (SF?9), encodes a 632-aa isoform lacking transmembrane and cytoplasmic domains, and its authentic expression was verified by PCR and Western blotting. SF?9 is expressed on the cell surface but cannot bind ligand in the absence of the full-length ?9 subunit. Over-expression of SF?9 in cells expressing full-length ?9 promotes ?9-dependent cell adhesion. This promoting effect of SF?9 requires the authentic cytoplasmic domain of the co-expressed full-length ?9 subunit. Thus, SF?9 is a novel functional modulator of ?9?1 integrin by inside-out signaling.

SUBMITTER: Kon S

PROVIDER: S-EPMC3118859 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature

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Short form of α9 promotes α9β1 integrin-dependent cell adhesion by modulating the function of the full-length α9 subunit.

Kon Shigeyuki S Atakilit Amha A Sheppard Dean D

Experimental cell research 20110416 12

The α9β1 integrin is a multifunctional receptor that interacts with a variety of ligands including vascular cell adhesion molecule 1, tenascin-C, and osteopontin. A 2.3-kb truncated form of α9 integrin subunit cDNA was identified by searching the Medline database. This splice variant, which we called the short form of α9 integrin (SFα9), encodes a 632-aa isoform lacking transmembrane and cytoplasmic domains, and its authentic expression was verified by PCR and Western blotting. SFα9 is expressed ...[more]

PMID: 21515257

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