Ontology highlight
ABSTRACT:
SUBMITTER: Brown HF
PROVIDER: S-EPMC3120127 | biostudies-literature | 2011 Jul-Aug
REPOSITORIES: biostudies-literature
Molecular and biochemical parasitology 20110420 1-2
Use of the yeast two-hybrid assay to study Plasmodium falciparum protein-protein interactions is limited by poor expression of P. falciparum genes in yeast and lack of easily implemented assays to confirm the results. We report here two methods to create gene fragments - random fragmentation by partial DNAse I digestion and generation of densely overlapping fragments by PCR - that enable most portions of P. falciparum genes to be expressed and screened in the yeast two-hybrid assay. The PCR-base ...[more]