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Yeast two-hybrid screens: improvement of array-based screening results by N- and C-terminally tagged fusion proteins.

ABSTRACT: Matrix-based yeast two-hybrid screens are an alternative to library-based screens. Recent improvements of matrix screens (also called array screens), use various pooling strategies as well as novel vectors that increase its efficiency while decreasing the false-negative rate, thus increasing reliability. In this chapter, we describe a screening strategy that systematically combines N- and C-terminal fusion proteins using a recently developed vector system.

SUBMITTER: Stellberger T

PROVIDER: S-EPMC7120008 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

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Yeast two-hybrid screens: improvement of array-based screening results by N- and C-terminally tagged fusion proteins.

Stellberger Thorsten T Häuser Roman R Uetz Peter P Brunn Albrecht von Av

Methods in molecular biology (Clifton, N.J.) 20120101

Matrix-based yeast two-hybrid screens are an alternative to library-based screens. Recent improvements of matrix screens (also called array screens), use various pooling strategies as well as novel vectors that increase its efficiency while decreasing the false-negative rate, thus increasing reliability. In this chapter, we describe a screening strategy that systematically combines N- and C-terminal fusion proteins using a recently developed vector system. ...[more]

PMID: 22130999

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