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A sequential strand-displacement strategy enables efficient six-step DNA-templated synthesis.

ABSTRACT: We developed a sequential strand-displacement strategy for multistep DNA-templated synthesis (DTS) and used it to mediate an efficient six-step DTS that proceeded in 35% overall yield (83% average yield per step). The efficiency of this approach and the fact that the final product remains linked to a DNA sequence that fully encodes its reaction history suggests its utility for the translation of DNA sequences into high-complexity synthetic libraries suitable for in vitro selection.

SUBMITTER: He Y

PROVIDER: S-EPMC3125949 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature

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A sequential strand-displacement strategy enables efficient six-step DNA-templated synthesis.

He Yu Y Liu David R DR

Journal of the American Chemical Society 20110614 26

We developed a sequential strand-displacement strategy for multistep DNA-templated synthesis (DTS) and used it to mediate an efficient six-step DTS that proceeded in 35% overall yield (83% average yield per step). The efficiency of this approach and the fact that the final product remains linked to a DNA sequence that fully encodes its reaction history suggests its utility for the translation of DNA sequences into high-complexity synthetic libraries suitable for in vitro selection. ...[more]

PMID: 21657248

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