Ontology highlight
ABSTRACT:
SUBMITTER: Nagel ZD
PROVIDER: S-EPMC3127881 | biostudies-literature | 2011 Jun
REPOSITORIES: biostudies-literature
Nagel Zachary D ZD Dong Ming M Bahnson Brian J BJ Klinman Judith P JP
Proceedings of the National Academy of Sciences of the United States of America 20110613 26
A growing body of data supports a role for protein motion in enzyme catalysis. In particular, the ability of enzymes to sample catalytically relevant conformational substates has been invoked to model kinetic and spectroscopic data. However, direct experimental links between rapidly interconverting conformations and the chemical steps of catalysis remain rare. We report here on the kinetic analysis and characterization of the hydride transfer step catalyzed by a series of mutant thermophilic alc ...[more]