Unknown

Dataset Information

0

Impaired protein conformational landscapes as revealed in anomalous Arrhenius prefactors.


ABSTRACT: A growing body of data supports a role for protein motion in enzyme catalysis. In particular, the ability of enzymes to sample catalytically relevant conformational substates has been invoked to model kinetic and spectroscopic data. However, direct experimental links between rapidly interconverting conformations and the chemical steps of catalysis remain rare. We report here on the kinetic analysis and characterization of the hydride transfer step catalyzed by a series of mutant thermophilic alcohol dehydrogenases (ht-ADH), presenting evidence for Arrhenius prefactor values that become enormously elevated above an expected value of approximately 10(13) s(-1) when the enzyme operates below its optimal temperature range. Restoration of normal Arrhenius behavior in the ht-ADH reaction occurs at elevated temperatures. A simple model, in which reduced temperature alters the ability of the ht-ADH variants to sample the catalytically relevant region of conformational space, can reproduce the available data. These findings indicate an impaired landscape that has been generated by the combined condition of reduced temperature and mutation at a single, active-site hydrophobic side chain. The broader implication is that optimal enzyme function requires the maintenance of a relatively smooth landscape that minimizes low energy traps.

SUBMITTER: Nagel ZD 

PROVIDER: S-EPMC3127881 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature

altmetric image

Publications

Impaired protein conformational landscapes as revealed in anomalous Arrhenius prefactors.

Nagel Zachary D ZD   Dong Ming M   Bahnson Brian J BJ   Klinman Judith P JP  

Proceedings of the National Academy of Sciences of the United States of America 20110613 26


A growing body of data supports a role for protein motion in enzyme catalysis. In particular, the ability of enzymes to sample catalytically relevant conformational substates has been invoked to model kinetic and spectroscopic data. However, direct experimental links between rapidly interconverting conformations and the chemical steps of catalysis remain rare. We report here on the kinetic analysis and characterization of the hydride transfer step catalyzed by a series of mutant thermophilic alc  ...[more]

Similar Datasets

| S-EPMC7021514 | biostudies-literature
| S-EPMC4706811 | biostudies-literature
| S-EPMC4117682 | biostudies-literature
| S-EPMC3525846 | biostudies-other
| S-EPMC5441784 | biostudies-literature
| S-EPMC6557943 | biostudies-literature
| S-EPMC4629913 | biostudies-literature
| S-EPMC6773748 | biostudies-literature
2024-10-14 | GSE271825 | GEO
| S-EPMC7045699 | biostudies-literature