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Antagonistic regulation of EMT by TIF1? and Smad4 in mammary epithelial cells.


ABSTRACT: TGF-? is a potent inducer of epithelial-to-mesenchymal transition (EMT), a process involved in tumour invasion. TIF1? participates in TGF-? signalling. To understand the role of TIF1? in TGF-? signalling and its requirement for EMT, we analysed the TGF-?1 response of human mammary epithelial cell lines. A strong EMT increase was observed in TIF1?-silenced cells after TGF-?1 treatment, whereas Smad4 inactivation completely blocked this process. Accordingly, the functions of several TIF1? target genes can be linked to EMT, as shown by microarray analysis. As a negative regulator of Smad4, TIF1? could be crucial for the regulation of TGF-? signalling. Furthermore, TIF1? binds to and represses the plasminogen activator inhibitor 1 promoter, demonstrating a direct role of TIF1? in TGF-?-dependent gene expression. This study shows the molecular relationship between TIF1? and Smad4 in TGF-? signalling and EMT.

SUBMITTER: Hesling C 

PROVIDER: S-EPMC3128966 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature

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TGF-β is a potent inducer of epithelial-to-mesenchymal transition (EMT), a process involved in tumour invasion. TIF1γ participates in TGF-β signalling. To understand the role of TIF1γ in TGF-β signalling and its requirement for EMT, we analysed the TGF-β1 response of human mammary epithelial cell lines. A strong EMT increase was observed in TIF1γ-silenced cells after TGF-β1 treatment, whereas Smad4 inactivation completely blocked this process. Accordingly, the functions of several TIF1γ target g  ...[more]

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