Differential requirement for the dual functions of ?-catenin in embryonic stem cell self-renewal and germ layer formation.

ABSTRACT: Canonical Wnt signalling has been implicated in mouse and human embryonic stem cell (ESC) maintenance; however, its requirement is controversial. ?-catenin is the key component in this highly conserved Wnt pathway, acting as a transcriptional transactivator. However, ?-catenin has additional roles at the plasma membrane regulating cell-cell adhesion, complicating the analyses of cells/tissues lacking ?-catenin. We report here the generation of a Ctnnb1 (?-catenin)-deficient mouse ESC (mESC) line and show that self-renewal is maintained in the absence of ?-catenin. Cell adhesion is partially rescued by plakoglobin upregulation, but fails to be maintained during differentiation. When differentiated as aggregates, wild-type mESCs form descendants of all three germ layers, whereas mesendodermal germ layer formation and neuronal differentiation are defective in Ctnnb1-deficient mESCs. A Tcf/Lef-signalling-defective ?-catenin variant, which re-establishes cadherin-mediated cell adhesion, rescues definitive endoderm and neuroepithelial formation, indicating that the ?-catenin cell-adhesion function is more important than its signalling function for these processes.

SUBMITTER: Lyashenko N 

PROVIDER: S-EPMC3130149 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature