Majority of CD4+ T cells from peripheral blood of HIV-1-infected individuals contain only one HIV DNA molecule.
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ABSTRACT:
SUBMITTER: Josefsson L
PROVIDER: S-EPMC3131354 | biostudies-literature | 2011 Jul
REPOSITORIES: biostudies-literature
Publications
Majority of CD4+ T cells from peripheral blood of HIV-1-infected individuals contain only one HIV DNA molecule.
Josefsson Lina L King Martin S MS Makitalo Barbro B Brännström Johan J Shao Wei W Maldarelli Frank F Kearney Mary F MF Hu Wei-Shau WS Chen Jianbo J Gaines Hans H Mellors John W JW Albert Jan J Coffin John M JM Palmer Sarah E SE
Proceedings of the National Academy of Sciences of the United States of America 20110620 27
Neither the number of HIV-1 proviruses within individual infected cells in HIV-1-infected patients nor their genetic relatedness within individual infected cells and between cells and plasma virus are well defined. To address these issues we developed a technique to quantify and genetically characterize HIV-1 DNA from single infected cells in vivo. Analysis of peripheral blood CD4(+) T cells from nine patients revealed that the majority of infected cells contain only one copy of HIV-1 DNA, imply ...[more]
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