S. pombe genome deletion project: an update.
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ABSTRACT:
SUBMITTER: Spirek M
PROVIDER: S-EPMC3132452 | biostudies-literature | 2010 Jun
REPOSITORIES: biostudies-literature
Publications
S. pombe genome deletion project: an update.
Spirek Mario M Benko Zsigmond Z Carnecka Martina M Rumpf Cornelia C Cipak Lubos L Batova Monika M Marova Ivana I Nam Miyoung M Kim Dong-Uk DU Park Han-Oh HO Hayles Jacqueline J Hoe Kwang-Lae KL Nurse Paul P Gregan Juraj J
Cell cycle (Georgetown, Tex.) 20100615 12
The fission yeast Schizosaccharomyces pombe is a model organism used widely to study various aspects of eukaryotic biology. A collection of heterozygous diploid strains containing individual deletions in nearly all S. pombe genes has been created using a PCR based strategy. However, deletion of some genes has not been possible using this methodology. Here we use an efficient knockout strategy based on plasmids that contain large regions homologous to the target gene to delete an additional 29 ge ...[more]
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