Characterization of peptide chain length and constituency requirements for YejABEF-mediated uptake of microcin C analogues.
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ABSTRACT:
SUBMITTER: Vondenhoff GH
PROVIDER: S-EPMC3133342 | biostudies-literature | 2011 Jul
REPOSITORIES: biostudies-literature
Publications
Characterization of peptide chain length and constituency requirements for YejABEF-mediated uptake of microcin C analogues.
Vondenhoff Gaston H M GH Blanchaert Bart B Geboers Sophie S Kazakov Teymur T Datsenko Kirill A KA Wanner Barry L BL Rozenski Jef J Severinov Konstantin K Van Aerschot Arthur A
Journal of bacteriology 20110520 14
Microcin C (McC), a natural antibacterial compound consisting of a heptapeptide attached to a modified adenosine, is actively taken up by the YejABEF transporter, after which it is processed by cellular aminopeptidases, releasing the nonhydrolyzable aminoacyl adenylate, an inhibitor of aspartyl-tRNA synthetase. McC analogues with variable length of the peptide moiety were synthesized and evaluated in order to characterize the substrate preferences of the YejABEF transporter. It was shown that a ...[more]
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