Ontology highlight
ABSTRACT:
SUBMITTER: Agafonov DE
PROVIDER: S-EPMC3133382 | biostudies-literature | 2011 Jul
REPOSITORIES: biostudies-literature
Agafonov Dmitry E DE Deckert Jochen J Wolf Elmar E Odenwälder Peter P Bessonov Sergey S Will Cindy L CL Urlaub Henning H Lührmann Reinhard R
Molecular and cellular biology 20110502 13
More than 200 proteins associate with human spliceosomes, but little is known about their relative abundances in a given spliceosomal complex. Here we describe a novel two-dimensional (2D) electrophoresis method that allows separation of high-molecular-mass proteins without in-gel precipitation and thus without loss of protein. Using this system coupled with mass spectrometry, we identified 171 proteins altogether on 2D maps of stage-specific spliceosomal complexes. By staining with a fluorescen ...[more]