Ontology highlight
ABSTRACT:
SUBMITTER: Gulyani A
PROVIDER: S-EPMC3135387 | biostudies-literature | 2011 Jun
REPOSITORIES: biostudies-literature
Gulyani Akash A Vitriol Eric E Allen Richard R Wu Jianrong J Gremyachinskiy Dmitriy D Lewis Steven S Dewar Brian B Graves Lee M LM Kay Brian K BK Kuhlman Brian B Elston Tim T Hahn Klaus M KM
Nature chemical biology 20110612 7
Fluorescent biosensors for living cells currently require laborious optimization and a unique design for each target. They are limited by the availability of naturally occurring ligands with appropriate target specificity. Here we describe a biosensor based on an engineered fibronectin monobody scaffold that can be tailored to bind different targets via high-throughput screening. We made this Src-family kinase (SFK) biosensor by derivatizing a monobody specific for activated SFKs with a bright d ...[more]