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Biosensor-aided high-throughput screening of hyper-producing cells for malonyl-CoA-derived products.


ABSTRACT: Malonyl-coenzyme A (CoA) is an important biosynthetic precursor in vivo. Although Escherichia coli is a useful organism for biosynthetic applications, its malonyl-CoA level is too low.To identify strains with the best potential for enhanced malonyl-CoA production, we developed a whole-cell biosensor for rapidly reporting intracellular malonyl-CoA concentrations. The biosensor was successfully applied as a high-throughput screening tool for identifying targets at a genome-wide scale that could be critical for improving the malonyl-CoA biosynthesis in vivo. The mutant strains selected synthesized significantly higher titers of the type III polyketide triacetic acid lactone (TAL), phloroglucinol, and free fatty acids compared to the wild-type strain, using malonyl-CoA as a precursor.These results validated this novel whole-cell biosensor as a rapid and sensitive malonyl-CoA high-throughput screening tool. Further analysis of the mutant strains showed that the iron ion concentration is closely related to the intracellular malonyl-CoA biosynthesis.

SUBMITTER: Li H 

PROVIDER: S-EPMC5669015 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature

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Biosensor-aided high-throughput screening of hyper-producing cells for malonyl-CoA-derived products.

Li Heng H   Chen Wei W   Jin Ruinan R   Jin Jian-Ming JM   Tang Shuang-Yan SY  

Microbial cell factories 20171102 1


<h4>Background</h4>Malonyl-coenzyme A (CoA) is an important biosynthetic precursor in vivo. Although Escherichia coli is a useful organism for biosynthetic applications, its malonyl-CoA level is too low.<h4>Results</h4>To identify strains with the best potential for enhanced malonyl-CoA production, we developed a whole-cell biosensor for rapidly reporting intracellular malonyl-CoA concentrations. The biosensor was successfully applied as a high-throughput screening tool for identifying targets a  ...[more]

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