Biocompatible artificial DNA linker that is read through by DNA polymerases and is functional in Escherichia coli.
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ABSTRACT:
SUBMITTER: El-Sagheer AH
PROVIDER: S-EPMC3136318 | biostudies-literature | 2011 Jul
REPOSITORIES: biostudies-literature
Publications
Biocompatible artificial DNA linker that is read through by DNA polymerases and is functional in Escherichia coli.
El-Sagheer Afaf H AH Sanzone A Pia AP Gao Rachel R Tavassoli Ali A Brown Tom T
Proceedings of the National Academy of Sciences of the United States of America 20110627 28
A triazole mimic of a DNA phosphodiester linkage has been produced by templated chemical ligation of oligonucleotides functionalized with 5'-azide and 3'-alkyne. The individual azide and alkyne oligonucleotides were synthesized by standard phosphoramidite methods and assembled using a straightforward ligation procedure. This highly efficient chemical equivalent of enzymatic DNA ligation has been used to assemble a 300-mer from three 100-mer oligonucleotides, demonstrating the total chemical synt ...[more]
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