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ABSTRACT:
SUBMITTER: Chen X
PROVIDER: S-EPMC3136661 | biostudies-literature | 2007 Aug
REPOSITORIES: biostudies-literature
Structure (London, England : 1993) 20070801 8
We report a normal-mode method for anisotropic refinement of membrane-protein structures, based on a hypothesis that the global near-native-state disordering of membrane proteins in crystals follows low-frequency normal modes. Thus, a small set of modes is sufficient to represent the anisotropic thermal motions in X-ray crystallographic refinement. By applying the method to potassium channel KcsA at 3.2 A, we obtained a structural model with an improved fit with the diffraction data. Moreover, t ...[more]